|
KMID : 0545119960060020128
|
|
Journal of Microbiology and Biotechnology
1996 Volume.6 No. 2 p.128 ~ p.131
|
|
|
Growth of Lactobacillus acidophilus in Whey-based Medium and Preparation of Cell Concentrate for Production of Probiotics
|
|
Hong, Seok-San
Kim, Wang-June/Cha, Seong-Kwan/Lee, Byong H.
|
|
|
Abstract
|
|
|
|
Lactobacillus acidophilus KFRI 233 (of human origin) exhibited a high tolerance to bile. The maximum cell yield was 6.6×10 exp (9) CFU per gram of whey in a 5.0% whey medium. Cell growth was improved with the addition of 0.5% thiotone and 0.25% calcium carbonate. Cell growth reached a maximum level of 5.4×10 exp (8) CFU/㎖ at 20 h. Eighty-nine percent of the viable cells in the centrifuged concentrate survived freezing at -70℃ and this frozen concentrate showed no reduction in the viable cell count after 30 days at -70℃. Eight percent of the viable cells survived freeze-drying after the addition of 1 g/ℓ sodium carbonate before harvesting by centrifuging and this freeze-dried concentrate showed only a slight reduction in the viable cell count after 30 days at 4℃.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|